Sampdoria's hopes of beating the drop were killed off on Sunday as they were relegated from Serie A.
Inter Milan strengthened their grip on second spot in the Serie A table with a 3-1 win over Fiorentina.
Roma midfielders Daniele De Rossi and Simone Perrotta have been handed three-game bans for the recent red cards.
AC Milan moved to within a point of the Serie A title after a 1-0 win over Bologna.
AC Milan won to extend their lead at the top of Serie A while Inter Milan have leapfrogged Napoli into second.
Lazio miss crucial chance while Cesena, Chievo and Genoa all win and Fiorentina and Lecce are held in Serie A.
Napoli kept the pressure on leaders AC Milan with a 2-0 win at Bologna, although the Rossoneri also won.
A busy afternoon in Serie A saw Napoli come back to beat Lazio, but top-four hopefuls Udinese fell to Lecce.
Luigi De Canio is confident Lecce can pick up the points they need to avoid relegation from Serie A.
Francesco Totti scored his 200th Serie A goal on Sunday, while Udinese maintained their recent fine form.
Leaders AC Milan were held at home by bottom club Bari and Francesco Totti settled the Rome derby.
David Pizarro slotted home a last-minute penalty for Roma on Friday to edge them to a 2-1 win over Lecce.
Udinese produced one of the results of the season as they hammered Palermo 7-0, while Inter beat Sampdoria.
Milan kept their title bid on track as Roma's collapse led to Claudio Ranieri handing in his resignation.
Lazio, Udinese and Juventus offered their respective top-four bids another welcome shot in the arm on Sunday.
Napoli and Inter Milan closed the gap on leaders AC Milan as Lazio endured another frustrating afternoon.
Napoli missed the chance to cut the gap on leaders AC Milan at the top, while Roma also failed to win.
Napoli moved back to within four points of leaders Milan after convincing 4-0 win over Sampdoria.
AC Milan moved four points clear at the top of Serie A with a 2-0 win over Cesena at the San Siro.
Leaders AC Milan were held to a surprise draw as Lazio, Roma and Juventus all secured late wins.
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