Conor O'Shea has stuck with the majority of his autumn international squad in naming his 32-man party for the Six Nations.
Two players from Zebre, tighthead prop Dario Chistolini and uncapped second row Federico Ruzza have been called up by the former Harlequins coach, who took over just after last year's tournament.
Chistolini replaces Benetton Treviso's Simone Ferrari, who is injured, while Azzurri great Martin Castrogiovanni has retired from international rugby since Italy's last match of the autumn.
"The group that we've seen in November has worked very well. For us it is important to give continuity to our work and increase the experience of the squad," O'Shea said.
"I'm looking forward to getting back to working with the guys and I expect a squad that will work hard to play every match until the last minute."
The Italy squad will meet from January 22 to 25 then reassemble four days later ahead of the start of this year's tournament, which they kick off against Wales on February 5.
Forwards: Pietro Ceccarelli, Dario Chistolini, Lorenzo Cittadini, Andrea Lovotti, Sami Panico, Tommaso D'Apice, Ornel Gega, Leonardo Ghiraldini, George Fabio Biagi, Joshua Furno, Marco Fuser, Federico Ruzza, Andries Van Schalkwyk, Marco Barbini, Simone Favaro, Maxime Mata Mbanda, Francesco Minto, Sergio Parisse (c), Abraham Jurgens Steyn.
Backs: Giorgio Bronzini, Edoardo Gori, Marcello Violi, Tommaso Allan, Carlo Canna, Tommaso Benvenuti, Tommaso Boni, Michele Campagnaro, Luke McLean, Giulio Bisegni, Angelo Esposito, Giovambattista Venditti, Edoardo Padovani.