Two fans have been banned from all matches at Twickenham for two years following an investigation into anti-gay abuse aimed at referee Nigel Owens during a game.
The abuse occurred during the Test match between England and New Zealand, at Twickenham, on November 8 and came to light in a letter from a spectator published by The Guardian.
The RFU said the two people will pay £1,000 to a charity of Owens' choice.
Ian Ritchie, chief executive of the RFU, said: "While instances of this nature are exceptionally rare, the RFU takes rugby's values of teamwork, respect, enjoyment, discipline and sportsmanship very seriously".
Owens, who is gay, is widely regarded as one of rugby's top referees.