Chelsea have been drawn against Atletico Madrid in the Women's Champions League last 16 while Manchester City will take on Fiorentina.
WSL leaders Chelsea will come up against a familiar face in Toni Duggan with the England international having helped Atletico knock Manchester City out of the tournament at the same stage last season.
Emma Hayes' side, semi-finalists in 2018 and 2019, have been in stunning form over the past year, going on a record-breaking 33-game unbeaten run which was only recently ended by a shock home defeat to Brighton.
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Meanwhile, Gareth Taylor's City will also play their first leg at home, against a Fiorentina side that finished second in Serie A last season but is off the pace this term, lying fourth and 16 points behind pacesetters Juventus.
City, semi-finalists in 2017 and 2018, will also be buoyed by the fact the Italians were beaten 6-0 on aggregate by Arsenal in last season's competition.
Holders Lyon, who have won a record seven titles and made it five in a row last season, face Danish side Brondby.
The only other past champions still in the competition are Wolfsburg who face Norwegian side LSK Kvinner - the former club of interim England head coach Hege Riise
First legs are scheduled to be played on March 3 or 4, with the return fixtures expected to take place on March 10 or 11.
The final will be played at Gothenburg's Gamla Ullevi stadium on May 16.
Full draw as follows:
Wolfsburg (GER) vs LSK Kvinner (NOR)
Barcelona (ESP) vs Fortuna Hjorring (DEN)
Rosengard (SWE) vs St Polten (AUT)
BIIK-Kazygurt (KAZ) vs Bayern Munich (GER)
Manchester City (ENG) vs Fiorentina (ITA)
Sparta Pragye (CZE) vs Paris Saint-Germain (FRA)
Lyon (FRA) vs Brondby (DEN)
Chelsea (ENG) vs Atletico Madrid (ESP)
Watch the Women's Football Show on Friday at 6pm on Sky Sports Football.