Ryan Bertrand missed England training on Wednesday but is expected to be fit for the opening game of Euro 2016.
The Southampton full-back had treatment away from the main group, all of whom completed the session at Watford.
Bertrand will not be considered for selection for England's final warm-up match, against Portugal on Thursday night, but will travel with the squad to France.
"It is not a serious injury and we are pretty confident he will be fit for when we play Russia," said manager Roy Hodgson.
"It is an injury which will keep him out of tomorrow's game but it is not a long term concern at all."
Liverpool striker Daniel Sturridge, who has not featured in either of England's friendly matches in the build-up to the tournament so far, trained with the squad and could play against Portugal.
Arsenal's Jack Wilshere and Jordan Henderson of Liverpool both missed large parts of the domestic season through injury but also made it through the session and are in line for more game time at Wembley.
England open their Euro 2016 Group B campaign with a match against Russia in Marseille on Saturday June 11 before taking on Wales in Lens on Thursday June 16 and Slovakia in Saint-Etienne on Monday June 20.
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