Atletico Madrid's faint La Liga title hopes look to be over after they lost 2-0 at Athletic Bilbao on Saturday.
Inaki Williams put the hosts ahead in the 73rd and Kenan Kodro netted in the 85th to move Athletic up to ninth.
Atletico's loss came despite a line-up that included the attacking trio of Antoine Griezmann, Diego Costa and Alvaro Morata.
They had won five in a row before losing 3-0 to Juventus on Tuesday to squander their 2-0 advantage from the first leg in their Champions League last-16 clash.
Atletico remain seven points behind Barcelona, who travel to Real Betis on Sunday.
Real Madrid are two points behind second-placed Atletico after marking Zinedine Zidane's return with a 2-0 win over Celta Vigo.
Alaves moved closer to the final Champions League spot with a 3-1 win at bottom side Huesca, moving within a point of fourth-place Getafe, who visit Valencia on Sunday.
Argentine striker Jonathan Calleri scored twice and Swedish forward John Guidetti added another for Alaves, who are unbeaten in six games.