Sky Bet League Two favourites Portsmouth will begin the 2016/17 season at home to Carlisle, while Doncaster go to Accrington.
Paul Cook's side are set to go into the new season as the bookmakers' fancies for the title ahead of newly-relegated Doncaster, who begin with a tough trip to last season's fourth-placed finishers Accrington.
Both sides promoted from the National League have home games first up, with champions Cheltenham opening against Leyton Orient and play-off winners Grimsby hosting Morecambe.
Plymouth, who missed out on promotion to League One when they lost to AFC Wimbledon at Wembley, are at home to Luton.
Opening day fixtures:
Accrington Stanley v Doncaster Rovers
Blackpool v Exeter City
Cambridge United v Barnet
Cheltenham Town v Leyton Orient
Crawley Town v Wycombe Wanderers
Grimsby Town v Morecambe
Hartlepool United v Colchester United
Newport County v Mansfield Town
Plymouth Argyle v Luton Town
Portsmouth v Carlisle United
Stevenage v Crewe Alexandra
Yeovil Town v Notts County
Final day fixtures:
Blackpool v Leyton Orient
Colchester United v Yeovil Town
Crawley Town v Mansfield Town
Crewe Alexandra v Barnet
Exeter City v Carlisle United
Grimsby Town v Plymouth Argyle
Hartlepool United v Doncaster Rovers
Luton Town v Morecambe
Newport County v Notts County
Portsmouth v Cheltenham Town
Stevenage v Accrington Stanley
Wycombe Wanderers v Cambridge United
Sky Sports has exclusively live coverage of the Sky Bet EFL in 2016/17 including all the drama of the Sky Bet Play-Offs.